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Project Overview

Full Title
Fate and transport of pathogenic microorganisms and nutrients originating from livestock animal manures applied to agricultural lands

Location
 Wake County, NC

Cooperating Agencies
U.S. Environmental Protection Agency, National Risk Management Laboratory, Land Remediation and Pollution Control Division, Cincinnati, Ohio

Project Chief
Stephen Harden

Period of Project
October 2009 – September 2012

Team Members
Doug Smith
Scott Caldwell
Shane Rogers (EPA)

Science Topic
Agriculture and Water Quality

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Fate and transport of pathogens and nutrients from land-applied animal manures

This project was completed in 2012. These pages are for historical purposes only.

Reports

OFR 2009-1128
Reconnaissance of Organic Wastewater Compounds at a Concentrated Swine Feeding Operation in the North Carolina Coastal Plain, 2008
By Stephen L. Harden
Open-File Report 2009–1128
OFR 2008-1156
Microbial and nutrient concentration and load data during stormwater runoff at a swine concentrated animal feeding operation in the North Carolina Coastal Plain, 2006-2007
By Stephen L. Harden
Open-File Report 2008-1156
SIR 2008-5021
Factors affecting nitrate delivery to streams from shallow ground water in the North Carolina Coastal Plain
By S.L. Harden and T.B. Spruill
Scientific Investigations Report 2008-5021
SIR 2004-5283
Geochemistry and characteristics of nitrogen transport at a confined animal feeding operation in a Coastal Plain agricultural watershed, and implications for nutrient loading in the Neuse River Basin, North Carolina, 1999-2002
By T.B. Spruill, A.J. Tesoriero, H.E. Mew, Jr., K.M. Farrell, S.L. Harden, A.B. Colosimo, and S.R. Kraemer
Scientific Investigations Report 2004-5283

REFERENCES

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Brown, G.K., Zaugg, S.D., and Barber, L.B. 1999. Wastewater analysis by gas chromatography/mass spectrometry: in Morganwalp, D.W., and Buxton, H.T., eds., U.S. Geological Survey Toxic Substances Hydrology Program--Proceedings of the Technical Meeting, Charleston, South Carolina, March 8-12, 1999--Volume 2 of 3--Contamination of Hydrologic Systems and Related Ecosystems: U.S. Geological Survey Water-Resources Investigations Report 99-4018B, p. 431-435.

Chen, J., Z. Yu, F.C. Michel, T. Wittum, and M. Morrison. 2007. Development and Application of Real-Time PCR Assays for Quantification of erm Genes Conferring Resistance to Macrolides-Lincosamides-Streptogramin B in Livestock Manure and Manure Management Systems, Appl. Environ. Microbiol., 73(14):4407–4416.

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Dick, L.K. and K.G. Field, 2004. Rapid Estimation of Numbers of Fecal Bacteroidetes by Use of a Quantitative PCR Assay for 16S rRNA Genes. Appl. Environ. Microbiol., 70:5695-5697.

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Hirsch, R., T.A. Ternes, K. Haberer, A. Mehlich, F. Ballwanz, and K.L. Kratz. 1998. Determination of antibiotics in different water compartments via liquid chromatography-electrospray tandem mass spectrometry, J. Chromatography A, 518:213-223.

Ibekwe, A.M., P.M. Watt, C.M. Grieve, V.K. Sharma, and S.R. Lyons. 2002. Multiplex fluorogenic real-time PCR for detection and quantification of Escherichia coli O157:H7 in dairy wastewater wetlands, Appl. Environ. Microbiol., 68(10):4853-4862.

Kolpin, D.W., Furlong, E.T., Meyer, M.T., Thurman, E.M., Zaugg, S.D., Barber, L.B., and Buxton, H.T. 2002. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999- 2000: A national reconnaissance, Environ. Sci. Technol., 36(6):1202-1211.

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Lund, M., S. Nordentoft, K. Pedersen, and M. Madsen. 2004. Detection of Campylobacter spp. in Chicken Fecal Samples by Real-Time PCR, Journal of Clinical Microbiology, 42(11) 5125–5132.

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Meyer, M.T., Bumgarner, J.E., Varns, J.L., Daughtridge, J.V., Thurman, E.M., and Hostetler, K.A. 2000. Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography / mass spectrometry, The Science of the Total Environment, 248:181-187.

Mieszkin, S., J.P. Furet, G. Corthier, M. Gourmelon. 2009. Estimation of Pig Fecal Contamination in a River Catchment by Real-Time PCR Using Two Pig-Specific Bacteroidales 16S rRNA Genetic Markers, Appl. Environ. Microbiol., 75: 3045-3054.

Patton, C.J., Kryskalla, J.R., 2003, Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory—Evaluation of alkaline persulfate digestion as an alternative to Kjedahl digestion for determination of total and dissolved nitrogen and phosphorus in water: U.S. Geological Survey Water-Resources Investigation Report 03-4174, 33 p.

Pei, R., S.C. Kim, K. Carlson, and A. Pruden. 2006. Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG), Water Research, 40:2427-2435

Smith M.S., R.K. Yang, C.W. Knapp, Y. Niu, N. Peak, M.M. Hanfelt, J.C. Galland, and D.W. Graham .2004. Quantification of Tetracycline Resistance Genes in Feedlot Lagoons by Real-Time PCR, Appl. Environ. Microbiol., 70(12):7372–7377.

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